FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Is mRNA isolation necessary for sensitive RT-PCR?
mRNA isolation is not normally required. PCRBIO 1-Step Go RT-PCR Kit has been developed to work on samples containing as little as 1 pg total RNA or 0.01 pg mRNA.
If you’re working with rare mRNA species, use a sequence-specific primer in the RT reaction to increase the sensitivity.
