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What if the measurement I get with my NGSBIO Library Quant Kit does not agree with my Qubit, spectrophotometer, or another method measurement?

Instruments like the Qubit or a spectrophotometer measure the total amount of DNA present in solution. The same is true of concentration measurements by an Agilent Bioanalyser. The NGSBIO Library Quant Kit for Illumina® will only quantify molecules with both adapters present. In addition, qPCR is a much more accurate method in general for quantifying DNA compared to fluorescent or spectroscopic methods. It is normal for these different measurement methods to disagree.

If the concentration measured by qPCR is significantly lower than what is measured by another method, this suggests that only a small proportion of molecules correctly incorporated the adapters during adapter ligation.

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