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What if my R2 value is not between 0.99 and 1.00?

If the R2 value is not close to 1, this suggests that the standards are not spaced evenly. Inspect the standard curve chart and remove any points that are far from the line. As long as there are three standards with two measurements each, the quantification will be accurate.

If you are unable to bring the quality of the fit to the necessary level, the experiment should be repeated. Take extra care when pipetting the standards to ensure accurate volumes and prevent contamination.

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