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Browse our frequently asked technical questions by selecting a product below.
My standard curves are not evenly spaced. Does this matter?
If the overall efficiency of your standard curve is between 90% – 110% and your R2 value is close to 1, this is not a cause for concern. As long as the slope of the line through all of the standards is between 3.1 – 3.6, the quantification will be accurate.