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Browse our frequently asked technical questions by selecting a product below.
My samples fall outside of the range of standards. What troubleshooting suggestions do you have?
Examine the melt curve to ensure that there is a single peak. If you see multiple peaks, there may be contamination present or unligated adapters. Otherwise, if the samples appear before DNA Standard 1, dilute the sample 100x and repeat. If the samples appear after DNA Standard 5, use a sample that is 100x more concentrated.