FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
When performing a multiplex, what is the recommended concentration of each primer?
We recommend using 400-1000 nM of each primer. There is a degree of flexibility around this recommended concentration, however the primer concentration should not be increased beyond this range as this may significantly affect the activity of the enzyme. Normally the concentration of the probe is half of that of the amplifying primers, but optimisation and validation are required for each primer set.
