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FAQs

Browse our frequently asked technical questions by selecting a product below.

What should be considered when doing multiplex amplification with the Lyo-Ready Probe 1-Step Kit?

The primers should be designed to ensure that they have similar annealing temperatures, are specific to the target, and do not form primer dimers. The length of the reverse transcription phase can be extended to 10 minutes to ensure there is enough template for priming and amplification.

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