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FAQs

Browse our frequently asked technical questions by selecting a product below.

How do I handle the Lyo-Ready Probe 1-Step Kit?

The Lyo-Ready Probe 1-Step Kit contains 2 components: 4x Lyo-Ready Probe Mix and 3200x UltraScript Reverse Transcriptase (RTase). 1.25 μL of 3200x UltraScript RTase should be added for each mL of 4x Lyo-Ready Probe Mix used. Due to the high concentration of the RTase, the volume supplied is very small, so we recommend spinning the tube every time before pipetting the desired volume of enzyme.

Whilst the 2 components of the kit are stable and have a longer shelf life (see expiry date of each component), the 4x Lyo-Ready Probe 1-Step Mix (obtained by adding the 3200x UltraScript RTase to the 4x Lyo-Ready Probe Mix) should be used immediately or can be stored for up to 3 days at 4 °C. We therefore recommend preparing only the amount of 4x Lyo-Ready Probe 1-Step Mix required and to return the remaining separate components to storage at between -30 °C and -15 °C.

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