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Browse our frequently asked technical questions by selecting a product below.
Yes, the enzyme is active between 57°C and 65°C. We do not recommend using it at temperatures outside this range as the activity will drop.
Yes, both IsoFast™ Bst Polymerase and RTase Go can be inactivated by heating the tube at >80°C for 10 minutes.
Unfortunately no, as the reaction temperatures are too high for enzyme stability.
Yes, but it is too low for use in reverse transcription applications. For some primer sets and targets IsoFast™ Bst Polymerase may be used as a single-enzyme (RT/DNA polymerase), but we recommend using the dedicated reverse transcriptase RTase Go as indicated.
Yes. Both the buffer and the mix already contain dNTPs, so there is no need to order dNTPs separately.
The kit consists of the 2x IsoFast™ Bst Mix (including the amplification buffer, dNTPs, MgSO4, IsoFast™ Bst polymerase) and of RTase Go at concentrations optimized for reverse transcription isothermal amplification. The kit also contains one tube of 20x fluorescence dye that binds to dsDNA for real-time detection of products. The user only needs to add primers and target RNA.