FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can products generated with Clara® and Clara® Purple mixes be digested, cloned, and sequenced?
Yes, PCR products generated with these mixes have the same characteristics as PCR products generated with wild-type Taq polymerase. They may be sequenced or digested with restriction endonucleases using standard protocols. Products are 3′-d(A)-tailed and may be used for TA cloning or may be blunt-ended or digested with restriction enzymes prior to cloning. For best results, we recommend purifying the PCR products using any standard PCR clean-up kit.
