VeriFi™ Library Amplification Mix is the ideal solution for NGS library preparation workflows and challenging PCRs on targets with a broad range of GC content.
To ensure accuracy of the final sequencing data, proofreading polymerases used for NGS library enrichment must have certain key characteristics:
- High fidelity, to reduce PCR errors in nucleotide incorporation.
- Strong multiplexing capability, to amplify varying fragment lengths in a library.
- Low sequence-dependent bias, particularly when dealing with complex (GC/AT-rich, or repetitive) sequences.
VeriFi™ Library Amplification Mix is engineered with these features in mind and consistently gives more uniquely mapped reads when compared to competing products. It is powered by our market-leading VeriFi™ Polymerase, with AptaLock™ hot start technology, in a novel 2x mix format. The mix contains reaction buffer, dNTPs, Mg2+, and enhancers that minimise GC-bias and enable reliable library amplification for PCR-based sequencing workflows.
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