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Multiplex 1-step RT-qPCR assay for accurate and sensitive detection of SARS-CoV-2

qPCRBIO Probe 1-Step Virus Detect Cover Image
qPCRBIO Probe 1-Step Virus Detect Cover Image

Multiplex virus detection assays have the potential to save a considerable amount of time, effort and reagent use, however they are far harder to optimise, given the increased number of targets in one reaction.

This application note details a rapid and sensitive multiplex RT-qPCR assay for SARS-CoV-2 detection, and is designed to support efforts in developing effective high-throughput diagnostic testing for COVID-19. Complete the form to download your copy today.

You will learn

  • An optimised experimental setup for multiplex detection of SARS-CoV-2
  • Recommended primer and probe sequences and concentrations
  • Recommended master mix and qPCR cycling conditions





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