Multiplex virus detection assays have the potential to save a considerable amount of time, effort and reagent use, however they are far harder to optimise, given the increased number of targets in one reaction.
This application note details a rapid and sensitive multiplex RT-qPCR assay for SARS-CoV-2 detection, and is designed to support efforts in developing effective high-throughput diagnostic testing for COVID-19. Complete the form to download your copy today.
You will learn
- An optimised experimental setup for multiplex detection of SARS-CoV-2
- Recommended primer and probe sequences and concentrations
- Recommended master mix and qPCR cycling conditions