IsoFast® Hot Start Bst Polymerase Colour & Mix
IsoFast® Hot Start Bst Colour reagents are colourimetric isothermal amplification enzyme formulations that combine IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid positive/negative screening.
This colourimetric formulation is available as a preassembled mix for direct sample and primer input, or as a separate enzyme and colour buffer format for full assay customisation and setup flexibility. This isothermal amplification system enables PCR-free, direct, colour-based readout of DNA targets that is ideal for point-of-care diagnostics and field testing.
IsoFast® Hot Start Bst Colour reagents combine IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid screening. The enzyme is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. These hot start colourimetric versions are the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed with minimal background amplification. With IsoFast® Hot Start colour reagents, your isothermal amplification experiments will be faster, more efficient, and easier than ever in both diagnostic applications and research endeavours.
Features
- Fast colour readout for positive/negative testing
- AptaLock™ hot start for ultra-sensitive detection of DNA targets
- Rapid polymerisation for faster time to results (as little as 10 mins)
- Detect down to 3 target copies per μL
- Ideal for both cold and room temperature setup
- Improved speed and sensitivity for early target detection
- High activity at a broad range of temperatures from 55-70 °C
Applications
- Colourimetric Isothermal Amplification
- Colourimetric LAMP
- Positive/negative DNA testing
- Rapid target screening
- Point-of-care testing
Hot start isothermal amplification
Hot start is important in isothermal amplification techniques because it helps to prevent non-specific amplification and false-positive results. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60°C and 65°C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.
Why is hot start crucial in colourimetric isothermal amplification?
- Preventing non-specific amplification in Bst polymerase isothermal reactions: In isothermal amplification, it’s essential to ensure that the amplification process only targets the specific sequence of interest. Non-specific amplification can occur when primers bind to unintended regions of the template or when amplification enzymes prematurely initiate amplification. Hot start techniques involve inhibiting the amplification reaction until the reaction reaches the desired temperature. This prevents the formation of non-specific products during the initial stages of the reaction when the temperature is still below the optimal amplification temperature.
- Enhancing Bst polymerase isothermal amplification specificity: By preventing premature amplification, hot start methods increase the specificity of the isothermal amplification reaction. This means that the reaction is less likely to amplify non-target sequences, reducing the risk of false-positive results.
- Improving isothermal amplification sensitivity with Bst polymerase: Hot start techniques can also improve the sensitivity of isothermal amplification assays. By minimising non-specific amplification, more of the available reagents are reserved for amplifying the target sequence, increasing the efficiency of the reaction and the detection limit.
Documents
Product Flyers
Product Manuals
Material Safety Data Sheets
Certificate of Analysis Finder
Specifications
IsoFast® Hot Start Bst Polymerase Colour
Component
1600 Units
8000 Units
IsoFast Hot Start Bst Polymerase 8 U/μL
1 x 200 μL
1 x 1 mL
10x IsoFast Colour Buffer A
1 x 500 μL
2 x 1.25 mL
5x IsoFast Buffer B
1 x 1 mL
3 x 1.7 mL
IsoFast® Hot Start Bst Colour Mix
Component
100 Reactions
500 Reactions
2x IsoFast Hot Start Bst Colour Mix
1 x 1.25 mL
5 x 1.25 mL
IsoFast® Hot Start Bst Polymerase Colour
Component
IsoFast Hot Start Bst Polymerase 8 U/μL
10x IsoFast Colour Buffer A
5x IsoFast Buffer B
1600 Units
1 x 200 μL
1 x 500 μL
1 x 1 mL
8000 Units
1 x 1 mL
2 x 1.25 mL
3 x 1.7 mL
IsoFast® Hot Start Bst Colour Mix
Component
2x IsoFast Hot Start Bst Colour Mix
100 Reactions
1 x 1.25 mL
500 Reactions
5 x 1.25 mL
Reaction Volume
Storage
25 μL
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full until the indicated expiry date.
Reaction Volume
25 μL
Storage
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full until the indicated expiry date.
FAQs
Can I use products amplified with IsoFast® Hot Start Bst reagents in downstream applications, such as cloning or sequencing?
Certain types of isothermal amplification strategies lend themselves to downstream applications, e.g. RCA (rolling circle amplification) or WGA (whole genome amplification) for NGS. IsoFast® Hot Start Bst reagents can be used for this type of amplification. However, the most common type of isothermal amplification, LAMP, generates multiple species of DNA products, making it unsuitable for cloning and impractical for NGS applications.
What type of nucleic acid can I amplify with IsoFast® Hot Start Bst reagents?
IsoFast® Hot Start Bst Polymerase, IsoFast® Hot Start Bst Mix, IsoFast® Hot Start Bst Colour Mix, and IsoFast® Hot Start Bst Polymerase Colour are all designed for amplification of DNA and cDNA targets. They are not suitable for direct amplification of RNA targets.
In order to amplify RNA targets, we recommend using our IsoFast® Bst 1-Step Mix, for direct 1-step isothermal amplification. Alternatively, we suggest a 2-step format, where RNA is converted to cDNA using one of our UltraScript® reagents, followed by amplification of cDNA with the users’ preferred IsoFast® Hot Start Bst reagent.
IsoFast® Hot Start Bst Colour reagents combine IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid screening. The enzyme is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. These hot start colourimetric versions are the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed with minimal background amplification. With IsoFast® Hot Start colour reagents, your isothermal amplification experiments will be faster, more efficient, and easier than ever in both diagnostic applications and research endeavours.
Features
- Fast colour readout for positive/negative testing
- AptaLock™ hot start for ultra-sensitive detection of DNA targets
- Rapid polymerisation for faster time to results (as little as 10 mins)
- Detect down to 3 target copies per μL
- Ideal for both cold and room temperature setup
- Improved speed and sensitivity for early target detection
- High activity at a broad range of temperatures from 55-70 °C
Applications
- Colourimetric Isothermal Amplification
- Colourimetric LAMP
- Positive/negative DNA testing
- Rapid target screening
- Point-of-care testing
Hot start isothermal amplification
Hot start is important in isothermal amplification techniques because it helps to prevent non-specific amplification and false-positive results. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60°C and 65°C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.
Why is hot start crucial in colourimetric isothermal amplification?
- Preventing non-specific amplification in Bst polymerase isothermal reactions: In isothermal amplification, it’s essential to ensure that the amplification process only targets the specific sequence of interest. Non-specific amplification can occur when primers bind to unintended regions of the template or when amplification enzymes prematurely initiate amplification. Hot start techniques involve inhibiting the amplification reaction until the reaction reaches the desired temperature. This prevents the formation of non-specific products during the initial stages of the reaction when the temperature is still below the optimal amplification temperature.
- Enhancing Bst polymerase isothermal amplification specificity: By preventing premature amplification, hot start methods increase the specificity of the isothermal amplification reaction. This means that the reaction is less likely to amplify non-target sequences, reducing the risk of false-positive results.
- Improving isothermal amplification sensitivity with Bst polymerase: Hot start techniques can also improve the sensitivity of isothermal amplification assays. By minimising non-specific amplification, more of the available reagents are reserved for amplifying the target sequence, increasing the efficiency of the reaction and the detection limit.
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Your Selection:
Documents
Product Flyers
Product Manuals
Material Safety Data Sheets
Certificate of Analysis Finder
Specifications
IsoFast® Hot Start Bst Polymerase Colour
Component
1600 Units
8000 Units
IsoFast Hot Start Bst Polymerase 8 U/μL
1 x 200 μL
1 x 1 mL
10x IsoFast Colour Buffer A
1 x 500 μL
2 x 1.25 mL
5x IsoFast Buffer B
1 x 1 mL
3 x 1.7 mL
IsoFast® Hot Start Bst Colour Mix
Component
100 Reactions
500 Reactions
2x IsoFast Hot Start Bst Colour Mix
1 x 1.25 mL
5 x 1.25 mL
IsoFast® Hot Start Bst Polymerase Colour
Component
IsoFast Hot Start Bst Polymerase 8 U/μL
10x IsoFast Colour Buffer A
5x IsoFast Buffer B
1600 Units
1 x 200 μL
1 x 500 μL
1 x 1 mL
8000 Units
1 x 1 mL
2 x 1.25 mL
3 x 1.7 mL
IsoFast® Hot Start Bst Colour Mix
Component
2x IsoFast Hot Start Bst Colour Mix
100 Reactions
1 x 1.25 mL
500 Reactions
5 x 1.25 mL
Reaction Volume
Storage
25 μL
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full until the indicated expiry date.
Reaction Volume
25 μL
Storage
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full until the indicated expiry date.
FAQs
Can I use products amplified with IsoFast® Hot Start Bst reagents in downstream applications, such as cloning or sequencing?
Certain types of isothermal amplification strategies lend themselves to downstream applications, e.g. RCA (rolling circle amplification) or WGA (whole genome amplification) for NGS. IsoFast® Hot Start Bst reagents can be used for this type of amplification. However, the most common type of isothermal amplification, LAMP, generates multiple species of DNA products, making it unsuitable for cloning and impractical for NGS applications.
What type of nucleic acid can I amplify with IsoFast® Hot Start Bst reagents?
IsoFast® Hot Start Bst Polymerase, IsoFast® Hot Start Bst Mix, IsoFast® Hot Start Bst Colour Mix, and IsoFast® Hot Start Bst Polymerase Colour are all designed for amplification of DNA and cDNA targets. They are not suitable for direct amplification of RNA targets.
In order to amplify RNA targets, we recommend using our IsoFast® Bst 1-Step Mix, for direct 1-step isothermal amplification. Alternatively, we suggest a 2-step format, where RNA is converted to cDNA using one of our UltraScript® reagents, followed by amplification of cDNA with the users’ preferred IsoFast® Hot Start Bst reagent.
