FAQs

Rapid Extract PCR Kit was tested with the following list of tissues

• Mammalian cell cultures
• Animal tissues from a variety of organisms (Fish, lobster, Mice tails / ears, Zebrafish, Nematodes, Kidney, Hair Follicle)
• Insects like Drosophila, mosquitos, beetle (legs, wings)
• Buccal swabs
• Gram positive bacteria
• Fungal Mycelia
• Stool samples
• FFPE
• Blood¹
• Fish Scale clips

The following tissues can require a pre-wash step before starting the PCRBIO Rapid Extract protocol²

• Yeast^2,3 (pre-wash or digestion with zymolase to obtain spheroblasts)
• Feather⁴ (requires modification of the protocol see below )
• Stool sample⁵ (requires dilution series to get rid of inhibitors)

If you have a small amount of starting material, we recommend keeping the extracted DNA in the lowest volume possible and to avoid any further dilutions after inactivating the protease.

1. Batubara, A. et al. 2016. Study of BMP15 gene polymorphism in Boer, Kacang, and Boerka goats. Jurnal Ilmu Ternak Dan Veteriner 21(4): 224-230. DOI: http://dx.doi.org/10.14334/jitv.v21i4.1636 (2016).
2. Inglis, P.W. et al. 2018. Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high throughput SNP genotyping and sequencing Applications. PLOS ONE | https://doi.org/10.1371/journal.pone.0206085 October 18.
3. Suzuki, T. & Iwahashi, Y. 2013. RNA preparation of Saccharomyces cerevisiae using the digestion method may give misleading results. Appl Biochem Biotechnol 169:1620–1632 DOI 10.1007/s12010-012-0051-8
4. Bello, N. et al. 2001. Isolation of genomic DNA from feathers. J Vet Diagn Invest 13:162–164
5. Nantavisai, K. et al. 2007. Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalisin stool specimens. J. Clinical Microbiology. 45: 581–583  doi:10.1128/JCM.01823-06