FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
How can I use the SampleTrack buffer provided with this kit?
SampleTrack Buffer allows you to confirm that template has been added to each reaction. The template containing SampleTrack Buffer is yellow; when it is added to the blue qPCR master mix in a tube or well, the reaction turns green.
Add SampleTrack Buffer to each template sample before dispensing the template into the qPCR reactions. Do not add SampleTrack Buffer to the reaction master mix or as a separate component after template addition, as this will not allow you to track whether template has been added.
The required concentration of SampleTrack Buffer in the prepared template depends on the volume of template added to each reaction. Prepare sufficient template containing SampleTrack Buffer for all required reactions, ensuring that SampleTrack Buffer will be at a final concentration of 1x in the completed qPCR reaction.
The examples below provide sufficient prepared template for ten 20 µL reactions. This is sufficient for one target gene and one reference gene, each tested using four technical replicates, with additional volume allowed for pipetting.
| Template added per 20 µL reaction |
SampleTrack concentration required in prepared template |
Preparation for 10 reactions |
|---|---|---|
| 1 µL | 20x | 2 µL 100x SampleTrack Buffer + 8 µL template
10 µL total prepared template |
| 2 µL | 10x | 2 µL 100x SampleTrack Buffer + 18 µL template
20 µL total prepared template |
| 3 µL | 6.7x | 2 µL 100x SampleTrack Buffer + 28 µL template
30 µL total prepared template |
| 5 µL | 4x | 2 µL 100x SampleTrack Buffer + 48 µL template
50 µL total prepared template |
Mix the prepared template thoroughly before dispensing it into the qPCR plate. Dispense the template volume specified in the first column into each reaction. Use the same template volume in all reactions prepared from a given sample. If different template volumes are used, prepare each template with the corresponding concentration of SampleTrack Buffer. Scale all preparation volumes proportionally when more or fewer reactions are required.
