FAQs

Yes. miRNA can be extracted from all solid samples using PhaseAll™ and following the standard RNA isolation protocol described in the product manual. You can modify this protocol for extraction of free miRNAs from blood serum as outlined below:

1. Sample Preparation:
   Add 50–250 µl of serum to 750 µl PhaseAll™. Gently invert the tube 5–8 times and incubate at room temperature for 15 minutes.

2. Phase Separation:
   Add 200 µl chloroform, invert for 15 seconds, and incubate again for 5 minutes at room temperature.
   Centrifuge at 12,000 × g for 15 minutes at 4 °C.

3. RNA Precipitation:
   Carefully transfer the upper aqueous phase to a new tube. Add 500 µl isopropanol, incubate for 10 minutes at room temperature, and centrifuge again at 12,000 × g for 10 minutes at 4 °C.

4. RNA Washing and Resuspension:
   Wash the pellet with 75% ethanol, air-dry at room temperature for 30 minutes, and resuspend in 20 µl RNase-free water.

5. RNA Quantification:
   Measure RNA concentration and purity using a NanoDrop™ UV-Vis Spectrophotometer at A260/280 nm and A260/230 nm ratios.