SampleTrack Buffer
Optimise your workflow and eliminate handling errors with this yellow sample visualisation buffer. SampleTrack Buffer is a 100x aqueous solution of a yellow dye, suitable for sample visualisation during PCR and qPCR reaction setup.
SampleTrack Buffer can be used to monitor sample addition to any qPCR or PCR mix from PCR Biosystems, including Red endpoint mixes and cDNA synthesis kits. It is particularly recommended in combination with Blue and Purple qPCR products
SampleTrack buffer helps you keep track of sample addition during PCR and qPCR setup. The buffer contains a yellow dye has an absorbance maximum at ~410 nm, and is inert, meaning it does not inhibit PCR reactions and does not quench fluorescence of SYBR-type dyes or common qPCR probes at the recommended concentration.
Features
- Easy-to-see sample visualisation dye
- Inert, doesn’t affect PCR and qPCR reactions
- Non-quenching, doesn’t quench common qPCR dyes
- Compatibility with all PCR Biosystems PCR and qPCR products
- High concentration, doesn’t dilute your sample
Applications
- Endpoint PCR
- qPCR
- Visualisation of PCR sample addition
When using PCR Biosystems “Blue” mixes, the reaction colour will switch from blue to green, and when using “Purple” mixes, the reaction colour will switch from purple to orange, when adding samples with SampleTrack Buffer. Clear mixes will correspondingly turn yellow. “Red” endpoint mixes will turn bright orange.
Simply add the buffer to any DNA, RNA or cDNA sample so that it has a 1x concentration in the final PCR or qPCR reaction volume (add to 5x final concentration when using with “Red” endpoint mixes). The dye can be added to the entire sample prior to storage, or to an aliquot, depending on experimental requirements. There is no need to amend cycling conditions, when using SampleTrack Buffer.
Documents
Product Manuals
Material Safety Data Sheets
Certificate of Analysis Finder
Specifications
SampleTrack Buffer
Component
1 mL
100x SampleTrack Buffer
2 x 0.5 mL
SampleTrack Buffer
Component
100x SampleTrack Buffer
1 mL
2 x 0.5 mL
Reaction Volume
Storage
NA
On arrival the dye should be stored between -30 °C and -20 °C. The product can also be stored at 4 °C for 1 month.
Reaction Volume
NA
Storage
On arrival the dye should be stored between -30 °C and -20 °C. The product can also be stored at 4 °C for 1 month.
FAQs
How can I use SampleTrack Buffer with PCRBIO reagents?
SampleTrack Buffer allows you to confirm that template has been added to each reaction. The template containing SampleTrack Buffer is yellow; when it is added to the blue qPCR master mix in a tube or well, the reaction turns green, when added to purple mixes reactions turn orange, when added to red mixes (at 5x final concentration) reactions turn bright red – orange, and when used with clear mixes these become yellow.
Add SampleTrack Buffer to each template sample before dispensing the template into the PCR or qPCR reactions. Do not add SampleTrack Buffer to the reaction master mix or as a separate component after template addition, as this will not allow you to track whether template has been added.
The required concentration of SampleTrack Buffer in the prepared template depends on the volume of template added to each reaction. Prepare sufficient template containing SampleTrack Buffer for all required reactions, ensuring that SampleTrack Buffer will be at a final concentration of 1x in the completed qPCR or PCR reaction for clear, blue and purple mixes and at 5x concentration in red endpoint mixes.
The examples below provide sufficient prepared template for ten 20 µL reactions. This is sufficient for one target gene and one reference gene, each tested using four technical replicates, with additional volume allowed for pipetting. Adjust volumes accordingly for 25, and 50 μL reactions, depending on your application and which PCR Biosystems reagent you are using.
| Template added per 20 µL reaction |
Concentration required in template for 1x final concentration |
Preparation for 10 reactions at 1x final concentration |
Concentration required in template for 5x final concentration |
Preparation for 10 PCRBIO Red Mix reactions at 5x final concentration |
|---|---|---|---|---|
| 1 µL | 20x | 2 µL 100x SampleTrack Buffer + 8 µL template 10 µL total prepared template |
100x | Not applicable Each reaction requires 1 µL of 100x SampleTrack Buffer, leaving no volume for template + sample in 1 µL. |
| 2 µL | 10x | 2 µL 100x SampleTrack Buffer + 18 µL template 20 µL total prepared template |
50x | 10 µL 100x SampleTrack Buffer + 10 µL template 20 µL total prepared template |
| 3 µL | 6.7x | 2 µL 100x SampleTrack Buffer + 28 µL template 30 µL total prepared template |
33.3x | 10 µL 100x SampleTrack Buffer + 20 µL template 30 µL total prepared template |
| 5 µL | 4x | 2 µL 100x SampleTrack Buffer + 48 µL template 50 µL total prepared template |
20x | 10 µL 100x SampleTrack Buffer + 40 µL template 50 µL total prepared template |
Mix the prepared template thoroughly before dispensing it into the PCR plate or wells. Dispense the template volume specified in the first column into each reaction. Use the same template volume in all reactions prepared from a given sample. If different template volumes are used, prepare each template with the corresponding concentration of SampleTrack Buffer. Scale all preparation volumes proportionally when more or fewer reactions are required.
SampleTrack buffer helps you keep track of sample addition during PCR and qPCR setup. The buffer contains a yellow dye has an absorbance maximum at ~410 nm, and is inert, meaning it does not inhibit PCR reactions and does not quench fluorescence of SYBR-type dyes or common qPCR probes at the recommended concentration.
Features
- Easy-to-see sample visualisation dye
- Inert, doesn’t affect PCR and qPCR reactions
- Non-quenching, doesn’t quench common qPCR dyes
- Compatibility with all PCR Biosystems PCR and qPCR products
- High concentration, doesn’t dilute your sample
Applications
- Endpoint PCR
- qPCR
- Visualisation of PCR sample addition
When using PCR Biosystems “Blue” mixes, the reaction colour will switch from blue to green, and when using “Purple” mixes, the reaction colour will switch from purple to orange, when adding samples with SampleTrack Buffer. Clear mixes will correspondingly turn yellow. “Red” endpoint mixes will turn bright orange.
Simply add the buffer to any DNA, RNA or cDNA sample so that it has a 1x concentration in the final PCR or qPCR reaction volume (add to 5x final concentration when using with “Red” endpoint mixes). The dye can be added to the entire sample prior to storage, or to an aliquot, depending on experimental requirements. There is no need to amend cycling conditions, when using SampleTrack Buffer.
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Documents
Product Manuals
Material Safety Data Sheets
Certificate of Analysis Finder
Specifications
SampleTrack Buffer
Component
1 mL
100x SampleTrack Buffer
2 x 0.5 mL
SampleTrack Buffer
Component
100x SampleTrack Buffer
1 mL
2 x 0.5 mL
Reaction Volume
Storage
NA
On arrival the dye should be stored between -30 °C and -20 °C. The product can also be stored at 4 °C for 1 month.
Reaction Volume
NA
Storage
On arrival the dye should be stored between -30 °C and -20 °C. The product can also be stored at 4 °C for 1 month.
FAQs
How can I use SampleTrack Buffer with PCRBIO reagents?
SampleTrack Buffer allows you to confirm that template has been added to each reaction. The template containing SampleTrack Buffer is yellow; when it is added to the blue qPCR master mix in a tube or well, the reaction turns green, when added to purple mixes reactions turn orange, when added to red mixes (at 5x final concentration) reactions turn bright red – orange, and when used with clear mixes these become yellow.
Add SampleTrack Buffer to each template sample before dispensing the template into the PCR or qPCR reactions. Do not add SampleTrack Buffer to the reaction master mix or as a separate component after template addition, as this will not allow you to track whether template has been added.
The required concentration of SampleTrack Buffer in the prepared template depends on the volume of template added to each reaction. Prepare sufficient template containing SampleTrack Buffer for all required reactions, ensuring that SampleTrack Buffer will be at a final concentration of 1x in the completed qPCR or PCR reaction for clear, blue and purple mixes and at 5x concentration in red endpoint mixes.
The examples below provide sufficient prepared template for ten 20 µL reactions. This is sufficient for one target gene and one reference gene, each tested using four technical replicates, with additional volume allowed for pipetting. Adjust volumes accordingly for 25, and 50 μL reactions, depending on your application and which PCR Biosystems reagent you are using.
| Template added per 20 µL reaction |
Concentration required in template for 1x final concentration |
Preparation for 10 reactions at 1x final concentration |
Concentration required in template for 5x final concentration |
Preparation for 10 PCRBIO Red Mix reactions at 5x final concentration |
|---|---|---|---|---|
| 1 µL | 20x | 2 µL 100x SampleTrack Buffer + 8 µL template 10 µL total prepared template |
100x | Not applicable Each reaction requires 1 µL of 100x SampleTrack Buffer, leaving no volume for template + sample in 1 µL. |
| 2 µL | 10x | 2 µL 100x SampleTrack Buffer + 18 µL template 20 µL total prepared template |
50x | 10 µL 100x SampleTrack Buffer + 10 µL template 20 µL total prepared template |
| 3 µL | 6.7x | 2 µL 100x SampleTrack Buffer + 28 µL template 30 µL total prepared template |
33.3x | 10 µL 100x SampleTrack Buffer + 20 µL template 30 µL total prepared template |
| 5 µL | 4x | 2 µL 100x SampleTrack Buffer + 48 µL template 50 µL total prepared template |
20x | 10 µL 100x SampleTrack Buffer + 40 µL template 50 µL total prepared template |
Mix the prepared template thoroughly before dispensing it into the PCR plate or wells. Dispense the template volume specified in the first column into each reaction. Use the same template volume in all reactions prepared from a given sample. If different template volumes are used, prepare each template with the corresponding concentration of SampleTrack Buffer. Scale all preparation volumes proportionally when more or fewer reactions are required.
