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FAQs

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Can miRNA be amplified using UltraScript® cDNA Synthesis Kit?

miRNA should not be amplified using UltraScript® cDNA Synthesis Kit. UltraScript® cDNA Synthesis Kit Separate Oligos, UltraScript Reverse Transcriptase, or UltraScript® 2.0 Reverse Transcriptase should be used because they come with a buffer that does not contain random hexamers and oligod(T)s, which could interfere with miRNA specific primers.

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