FAQs
Get the most out of your enzyme with our documents, guides, brochures and tools
FAQs
Browse our frequently asked technical questions by selecting a product below.
How much ROX should I add to my qPCR reaction?
The dye is supplied at a 50 μM concentration that can be used as a 100x stock solution for instruments requiring high ROX concentration (500 nM) and as a 1000x stock for instruments requiring a low ROX concentration (50 nM). Use our qPCR Selection Tool to identify the appropriate ROX concentration for your instrument. If your instrument model is not listed, please refer to the manufacturer’s instructions or contact our technical support team.
PCR Biosystems supplies most qPCR products in premixed, separate tube and no-ROX formulations for maximum ease of use.
