FAQs
Get the most out of your enzyme with our documents, guides, brochures and tools
FAQs
Browse our frequently asked technical questions by selecting a product below.
How much magnesium chloride should I add to my PCR or qPCR reaction?
Further addition of PCR enhancers or MgCl2to the reaction is not recommended, as the buffer’s composition has been optimised to maximise PCR success rates. However, if developing a customised assay, additional MgCl2 should be added in 0.5 mM increments up to a maximum of 5-7 mM and results compared to unmodified reactions.
