FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
How much crude blood can I add per reaction?
We have successfully carried out multiplex reactions on up to 5 μL of blood sample (1.25 μL of crude blood diluted 1:4 in a NaCl/EDTA solution) per 20 μL reaction. This does lead to a reduction in overall fluorescence but does not inhibit qPCR reactions themselves, nor does it limit the sensitivity of the mix.
