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FAQs

Browse our frequently asked technical questions by selecting a product below.

How to use RiboShield™ RNase inhibitor?

All PCRBIO products for 1-step and 2-step RT-PCR and RT-qPCR, as well as for cDNA synthesis, already contain the RNase inhibitor, so there is no need to add RiboShield™ RNase inhibitor when using these products.

If used with other kits, we recommend adding 1µL (40U) of RiboShield™ RNase inhibitor in a reaction mix of 20µL. In case the source of RNA is known to be rich in RNases, a titration may be required to verify full protection of the ribonucleic acid.

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