FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Will the blue dye affect the probe intensity?
qPCRBIO Probe Blue Mix will necessarily lower the fluorescent intensity from probes by absorbing light at both the excitation and emission wavelengths. Refer to Table 1 in the qPCRBIO Probe Blue Mix manuals to see how much signal loss there is for certain fluorophores. If signal intensity is a concern, consider switching to a qPCRBIO Probe Mix without dye.
