FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Why are unsatisfactory or different results being observed when comparing qPCRBIO HRM Mix to a competitor mix?
HRM mixes from different manufacturers vary greatly in their ionic strength and enhancer composition. Cycling conditions that might work for one mix could be suboptimal for another and therefore a cycling program that works on one mix can’t be applied to another without optimisation. The cycling can be optimised by running gradients for each amplicon irrespective of calculated or theoretical annealing temperature.
If all the above has been applied and the results are still unsatisfactory, please email [email protected] and include the following information:
- Amplicon size
- Reaction setup
- Cycling conditions
- Screen grabs of amplification traces and melting profile
