FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
How can unspecific products be removed in HRM analysis?
Specificity can be increased by increasing the annealing temperature or redesigning primers. Ensure that there is no contaminating DNA as this could cause the unspecific signal to arise. When performing initial runs, we always recommend running gradients. We also recommend running agarose gel analysis, to fully troubleshoot the reaction and identify products.
