FAQs

qPCRBIO cDNA Synthesis Kit can amplify both eukaryotic and prokaryotic RNA. Our mix contains random hexamers that are universal primers for all types of RNA, provided it contains sufficiently long fragments. Due to the presence of hexamers, RNA from all organisms can be reverse transcribed if it is available for the RTase. There are also oligo-d(T) included in the mix, which work with A-tailed RNA such as eukaryotic mRNA.

For a more selective kit use UltraScript Reverse Transcriptase or UltraScript 2.0 Reverse Transcriptase as the buffer for these products come without any hexamers and oligo(dT)s. This allows you to determine your own priming strategy.