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FAQs

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Can miRNA be amplified using qPCRBIO cDNA Synthesis Kit?

miRNA can’t be amplified using qPCRBIO cDNA Synthesis Kit. UltraScript Reverse Transcriptase or UltraScript 2.0 Reverse Transcriptase should be used because they come with a buffer that does not contain random hexamers and oligod(T)s, which could interfere with miRNA specific primers.

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