FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can I use PCRBIO VeriFi Polymerase if my assay requires a specialized buffer?
The 5x VeriFi™ buffer supplied with VeriFi™ Polymerase has been developed specifically for this enzyme and we highly recommend using them together. However, VeriFi™ Polymerase should be compatible with any PCR buffer developed for use with Pfu polymerase. If you use a customised buffer with VeriFi™ Polymerase, keep in mind reaction parameters such as annealing temperature and concentrations of the enzyme, template, dNTPs and MgCl2, may require optimisation.
