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What is the recommended extension time for PCRBIO HS VeriFi™ Polymerase?

30 seconds per kilobase (kb) is recommended for amplification from eukaryotic DNA. In case the yield is lower than expected, this time could be increased to 40 sec/kb. We would not recommend decreasing the extension time to less than 30 sec/kb as this would significantly decrease the yield of product formation. Care should be taken in not loading too much template DNA. If there are non-specific bands present after amplification the amount of template DNA should be decreased. 2-step protocols which combine annealing and extension are also possible. Optimisation of the cycling conditions and template concentration may be required if you deviate from the conditions and concentration recommended in the manual.

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