FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
What is the difference between VeriFi™ Hot Start Polymerase and VeriFi™ Polymerase / PCRBIO HiFi Polymerase?
VeriFi™ Hot Start Polymerase is the hot start version of VeriFi™ Polymerase, and uses PCR Biosystems’ innovative AptaLock™ technology for increased sensitivity and specificity compared to the two non hot start enzymes. Reactions can also be set up at room temperature when using VeriFi™ Hot Start Polymerase.
Both VeriFi™ Hot Start Polymerase and VeriFi™ Polymerase have an enhanced processivity relative to PCRBIO HiFi Polymerase, resulting in higher yields and the ability to amplify longer (up to 17.5 kb) and more difficult targets. Both enzymes also have a higher fidelity than PCRBIO HiFi Polymerase.
Unlike PCRBIO HiFi polymerase, the VeriFi™ family of enzymes are also available as convenient 2x ready mixes with the option of a red dye for direct gel loading.
