FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
The high GC content in my DNA is causing low yields of PCR product. What is the optimal denaturation temperature and time for VeriFi™ Hot Start Polymerase in this case?
If you’re working with GC-rich DNA and finding the yield of your PCR product is too low, try increasing the denaturation temperature to 98-100 °C. VeriFi™ Hot Start Polymerase is capable of withstanding these temperatures and has shown increased yields for DNA with high GC content relative to denaturation at 95 °C. The denaturation time can be varied from 10 seconds up to 30 seconds if required.
Addition of 10x VeriMax Enhancer can also help to amplify GC-rich targets.
