FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Can I use VeriFi™ Hot Start Polymerase if my assay requires a specialised buffer?
The 5x VeriFi™ buffer supplied with VeriFi™ Hot Start Polymerase has been developed specifically for this enzyme and we highly recommend using them together. However, VeriFi™ Hot Start Polymerase should be compatible with any PCR buffer developed for use with Pfu polymerase. If you use a customised buffer with VeriFi™ Hot Start Polymerase, keep in mind that reaction parameters such as annealing temperature and concentrations of enzyme, template, dNTPs and MgCl2, may require optimisation.
