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When should IsoFast™ Bst Polymerase be the enzyme of choice?
IsoFast™ Bst Polymerase is a DNA polymerase with a very good strand displacement activity. As it has been isolated from the moderate thermophile Bacillus stearothermophilus (recently renamed as Geobacillus stearothermophilus), the temperature optimum of 60-65°C is higher than the one of DNA Polymerase I, Klenow Fragment or the one of phi29 polymerases (37°C), other frequently used strand displacing polymerases.
This is useful in the design of sequencing strategies as well as isothermal amplification technologies. For example, the higher reaction temperature facilitates sequencing through GC rich regions.