RiboShield™ RNase Inhibitor

RiboShield™ RNase Inhibitor is a recombinant protein that blocks the activity of a wide range of ribonucleases to reliably protect your RNA from RNase digestion.

The inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis and RNA sequencing, where the presence of even small amounts of RNase can be highly detrimental to RNA quality and experimental outcome. 

Features

  • Superior protection leading to better performance of RNA-sensitive applications
  • Particularly suited to incorporation into saliva-based tests for SARS-CoV-2 detection
  • Inhibits eukaryotic RNases, including RNase A, B and C
  • Compatible with reverse transcriptases, RNA polymerases and Taq DNA polymerase
  • Stable up to 65°C for at least 30 minutes
  • Ribonuclease and phosphatase free
  • Ideal for long term storage of samples
CAT No.
Product
Size
Price
QTY
PB30.23-02
RiboShield™ RNase Inhibitor
2500 Units
$103.00
PB30.23-10
RiboShield™ RNase Inhibitor
10 000 Units
$368.00

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More Information

Ribonucleases (RNases) are found in all cell types and organisms, and their ubiquitous nature can be highly problematic in the lab. Contamination with even the smallest amount of RNase in an RNA sample is enough to completely destroy the RNA. RiboShield™ RNase Inhibitor is a recombinant human placental protein that blocks the activity of a wide range of ribonucleases, including RNase A, B and C, to reliably protect your RNA from RNase digestion.

Superior RNA protection

RiboShield™ RNase Inhibitor is designed for RNA-sensitive applications including RNA isolation, RT-qPCR and RNA sequencing, to shield your RNA from degradation and provide higher yields and better performance as a result.

The inhibitor binds noncovalently to RNases at a 1:1 ratio and with very rapid kinetics of association, guaranteeing immediate protection of your RNA. RiboShield™ RNase Inhibitor has been tested in RT-qPCR and offers the greatest RNA protection when compared to leading competitor products (see figure 1).

Stability at higher temperatures

RiboShield™ RNase Inhibitor is able to perform over a wide range of reaction conditions and can sustain inhibition of RNase A at temperatures up to 65°C for at least 30 minutes (see figure 2). In addition, RiboShield™ does not contain cysteine residues that have been implicated in the oxidation sensitivity of the human placental version of the protein1. This results in an RNase inhibitor molecule that is not only thermostable, but also more resistant to oxidative stress.

Flexible

The inhibitor can be used to block the activity of a wide range of ribonucleases, including eukaryotic RNases of the neutral type (RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H. The inhibitor does not hinder other enzymes such as reverse transcriptases, RNA polymerases or Taq DNA polymerase making it compatible with many enzymatic reactions involving RNA.

RiboShield™ is purified from a strain of Pichia pastoris expressing a modified human placental gene. The inhibitor is free from ribonucleases and phophatases and is inactivated by heating at 75°C for 15 minutes.

1 Kim BM, Schultz LW, Raines RT. Variants of ribonuclease inhibitor that resist oxidation. Protein Science. 1999; 8(2):430-434. 

Applications

  • cDNA synthesis
  • 1-step RT-PCR
  • 1-step RT-qPCR
  • RNA purification
  • RNA sequencing
  • In vitro transcription and translation
  • Saliva-based diagnostic testing for SARS-CoV-2

Specifications

RiboShield™ RNase Inhibitor

Component

2500 Units

10 000 Units

RiboShield™ RNase Inhibitor (40U/µL)

1 x 62.5µL

4 x 62.5µL

RiboShield™ RNase Inhibitor

Component

RiboShield™ RNase Inhibitor (40U/µL)



2500 Units

1 x 62.5µL

10 000 Units

4 x 62.5µL

Reaction Volume

Storage

Not applicable

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Reaction Volume

Not applicable

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

FAQs

How to use RiboShield™ RNase inhibitor?

All PCRBIO products for 1-step and 2-step RT-PCR and RT-qPCR, as well as for cDNA synthesis, already contain the RNase inhibitor, so there is no need to add RiboShield™ RNase inhibitor when using these products.

If used with other kits, we recommend adding 1µL (40U) of RiboShield™ RNase inhibitor in a reaction mix of 20µL. In case the source of RNA is known to be rich in RNases, a titration may be required to verify full protection of the ribonucleic acid.

What can RiboShield™ RNase inhibitor be used for?

RiboShield™ RNase inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis and RNA-seq, where the presence of even small amounts of RNase can be detrimental to RNA quality and experimental outcome. In addition, it can be added to RNA extraction buffers to protect the ribonucleic acid from degradation at the first step of isolation.

What does RiboShield™ RNase inhibitor do?

RiboShield™ RNase inhibitor acts as a shield against the degradation of target RNA which can be caused by ribonuclease contamination of the RNA preparation.

What kind of RNases can be inhibited using RiboShield™ RNase inhibitor?

RiboShield™ RNase inhibitor is a protein-based inhibitor that non-covalently binds and blocks the activity of the most common and troublesome RNases, including eukaryotic RNases of the neutral type (e.g. RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.

More Information

Ribonucleases (RNases) are found in all cell types and organisms, and their ubiquitous nature can be highly problematic in the lab. Contamination with even the smallest amount of RNase in an RNA sample is enough to completely destroy the RNA. RiboShield™ RNase Inhibitor is a recombinant human placental protein that blocks the activity of a wide range of ribonucleases, including RNase A, B and C, to reliably protect your RNA from RNase digestion.

Superior RNA protection

RiboShield™ RNase Inhibitor is designed for RNA-sensitive applications including RNA isolation, RT-qPCR and RNA sequencing, to shield your RNA from degradation and provide higher yields and better performance as a result.

The inhibitor binds noncovalently to RNases at a 1:1 ratio and with very rapid kinetics of association, guaranteeing immediate protection of your RNA. RiboShield™ RNase Inhibitor has been tested in RT-qPCR and offers the greatest RNA protection when compared to leading competitor products (see figure 1).

Stability at higher temperatures

RiboShield™ RNase Inhibitor is able to perform over a wide range of reaction conditions and can sustain inhibition of RNase A at temperatures up to 65°C for at least 30 minutes (see figure 2). In addition, RiboShield™ does not contain cysteine residues that have been implicated in the oxidation sensitivity of the human placental version of the protein1. This results in an RNase inhibitor molecule that is not only thermostable, but also more resistant to oxidative stress.

Flexible

The inhibitor can be used to block the activity of a wide range of ribonucleases, including eukaryotic RNases of the neutral type (RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H. The inhibitor does not hinder other enzymes such as reverse transcriptases, RNA polymerases or Taq DNA polymerase making it compatible with many enzymatic reactions involving RNA.

RiboShield™ is purified from a strain of Pichia pastoris expressing a modified human placental gene. The inhibitor is free from ribonucleases and phophatases and is inactivated by heating at 75°C for 15 minutes.

1 Kim BM, Schultz LW, Raines RT. Variants of ribonuclease inhibitor that resist oxidation. Protein Science. 1999; 8(2):430-434. 

Applications

  • cDNA synthesis
  • 1-step RT-PCR
  • 1-step RT-qPCR
  • RNA purification
  • RNA sequencing
  • In vitro transcription and translation
  • Saliva-based diagnostic testing for SARS-CoV-2

Specifications

RiboShield™ RNase Inhibitor

Component

2500 Units

10 000 Units

RiboShield™ RNase Inhibitor (40U/µL)

1 x 62.5µL

4 x 62.5µL

RiboShield™ RNase Inhibitor

Component

RiboShield™ RNase Inhibitor (40U/µL)



2500 Units

1 x 62.5µL

10 000 Units

4 x 62.5µL

Reaction Volume

Storage

Not applicable

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Reaction Volume

Not applicable

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

FAQs

How to use RiboShield™ RNase inhibitor?

All PCRBIO products for 1-step and 2-step RT-PCR and RT-qPCR, as well as for cDNA synthesis, already contain the RNase inhibitor, so there is no need to add RiboShield™ RNase inhibitor when using these products.

If used with other kits, we recommend adding 1µL (40U) of RiboShield™ RNase inhibitor in a reaction mix of 20µL. In case the source of RNA is known to be rich in RNases, a titration may be required to verify full protection of the ribonucleic acid.

What can RiboShield™ RNase inhibitor be used for?

RiboShield™ RNase inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis and RNA-seq, where the presence of even small amounts of RNase can be detrimental to RNA quality and experimental outcome. In addition, it can be added to RNA extraction buffers to protect the ribonucleic acid from degradation at the first step of isolation.

What does RiboShield™ RNase inhibitor do?

RiboShield™ RNase inhibitor acts as a shield against the degradation of target RNA which can be caused by ribonuclease contamination of the RNA preparation.

What kind of RNases can be inhibited using RiboShield™ RNase inhibitor?

RiboShield™ RNase inhibitor is a protein-based inhibitor that non-covalently binds and blocks the activity of the most common and troublesome RNases, including eukaryotic RNases of the neutral type (e.g. RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.