At PCR Biosystems we understand that every molecular diagnostic assay is different
Whether the focus is infectious diseases, clinical oncology or point-of-care, we offer a comprehensive portfolio of molecular diagnostic reagents to simplify your R&D and power diagnostic development.
Our enzymes and mixes are extensively tested and undergo a comprehensive, multi-step quality control process according to ISO 13485 standards, to ensure optimum performance, consistency and traceability for your molecular diagnostic assay.
In addition we provide bulk and custom supply, OEM manufacturing, and tailored technical support to help you achieve the most from our market-leading reagents.
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We are PCR Biosystems
Lyophilisable and air-dryable reagents
Lyophilisation and air drying offers many advantages such as ambient storage and increased versatility in sample input volume. Our optimised lyo-ready mixes include a glycerol-free 4x qPCR mix and a blend of excipients for reliable lyophilisation, without loss of activity.
High concentration enzymes are also available to ensure you have complete control of your downstream assay development.
qPCR mixes for probe-based detection
Our universal probe mixes combine antibody-mediated hot start technology and advanced buffer chemistry to give superior sensitivity and specificity in all probe-based qPCR and 1-step RT-qPCR assays. The increased sensitivity of our probe-based qPCR mixes makes them the perfect choice for multiplexing diagnostic targets.
Taq polymerases and anti-Taq antibodies
Our enhanced Taq DNA polymerases are robust and versatile enzymes, provided with an optional 5x buffer that includes dNTPs, MgCl2 and enhancers. For hot start applications, PCRBIO HS Taq DNA Polymerase uses antibody-mediated hot start technology to maximise the sensitivity and specificity of your reaction.
We also provide our anti-Taq antibody blend as an individual component for enhanced assay optimisation.
Reverse Transcriptases for RT-qPCR assays
UltraScript and UltraScript 2.0 are thermostable modified MMLV reverse transcriptases engineered for superior cDNA synthesis speed, yield and representation. UltraScript allows highly sensitive reverse transcription up to 55 ˚C, whereas UltraScript 2.0 can be used with temperatures over 55 °C to tackle the most challenging RNA sample types.
Engineered for fast isothermal amplification, the IsoFast™ Bst range of reagents is based on the large fragment of Bst DNA polymerase. This portion of the enzyme contains 5’-3’ polymerase activity and strong strand displacement capabilities, with an optimal reaction temperature of 65 ˚C.