SARS-CoV-2 DetectionThe emergence and rapid spread of coronavirus disease 2019 (COVID-19) represents a major health threat to countries and populations around the world. The outbreak, originating in Wuhan, China, in December 2019 was declared a pandemic by the World Health Organisation (WHO) on 11th March 2020.
The causative agent of COVID-19 is the highly contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; previously 2019-nCoV), a betacoronavirus believed to have originated in bats, and spread in humans by direct contact with respiratory droplets.
Coronaviruses are enveloped, single-stranded, positive-sense RNA viruses that infect humans, but also a wide range of animals. SARS-CoV-2 infects human cells via S (spike) protein binding to ACE2 transmembrane receptors which are widely expressed in lung, heart, kidney and gastrointestinal tissue.
The emergence of new viral diseases such as COVID-19, and the closely related SARS in 2003 and MERS in 2012, highlights the need for rapid methods to detect and identify target viruses at scale. This page groups together our recommended RT-qPCR products for accurate and sensitive detection of the SARS-CoV-2 virus.
qPCRBIO Probe 1-Step Virus Detect
A high-concentration 4x RT-qPCR kit designed specifically for ultra-sensitive, high-throughput detection of viral RNA sequences, including SARS-CoV-2. Users can add more sample to their reactions to boost analytical sensitivity, even when working with small volume reactions.
qPCRBIO Probe 1-Step Go
This universal 2x master mix is engineered for fast and sensitive 1-step RT-qPCR using sequence-specific probes. The redesigned buffer system, thermostable reverse transcriptase and antibody-mediated hot start technology support rapid and accurate detection of the SARS-CoV-2 virus.
UltraScript Reverse Transcriptase
UltraScript is a thermostable reverse transcriptase, producing high quality cDNA ideal for a variety of downstream applications. The enzyme enables highly sensitive and accurate reverse transcription of viral RNA sequences, making it an ideal component of RT-qPCR-based SARS-CoV-2 detection tests.
PCRBIO HS Taq DNA Polymerase & Mixes
Whether you need a hot start enzyme for high-throughput assays, automated reaction setup or the detection of a low copy viral template, PCRBIO HS Taq DNA Polymerase is a robust, industry-leading enzyme to meet your needs. Available in standard and custom formats to suit the specific requirements of your COVID-19 diagnostic workflow.
qPCRBIO SyGreen 1-Step Kits
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.
qPCRBIO Probe Mix
qPCRBIO Probe Mix is the robust choice for all your probe-based real-time PCR needs. Our universal kit can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences.