Probe-based qPCR becomes a sensitive and rapid diagnostic tool for MPXV detection when combined with fast-cycling protocols.

This application note details how to use a multiplex probe-based qPCR assay, using Clara™ Probe Mix, with standard and fast cycling program setup to detect the Monkeypox (MPXV) virus and identify the viral strain. It is designed to support efforts in diagnosing this illness and can be used as a guide to setup multiplex assays for detection of any DNA virus or other DNA targets.

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You will find details on:

• Primer and probe sequences for MPXV generic and strain-specific detection
• Examples of standard and fast cycling qPCR program setup
• How fast cycling combined with sensitive probe chemistry make multiplex qPCR a rapid and powerful diagnostic tool